Microbial collection and Stabilization kits : OMNIgene®・GUT

OMNIgene® is a trademark of DNA Genotek Inc, Canada

Self-collection kits of microbial DNA from feces OMNIgene®・GUT

OMNIgene・GUT(OMR-200) can be easily taken the high quality sample at home rapidly homogenize and stabilize at the point of collection.

Transport and store stabilized DNA at ambient temperature for 60 days – no cold chain required.

OMNIgene Gut kit does not contain guanidine.

※For research

Features
What is microbiome?
Step1 Collect feces
Step2 Storage of samples
Step3 Purification of Samples
Step4 Downstream applications

Features

Fecal samples can be collected more easily than conventional methods (freeze).
--->Improve donor compliance with intuitive and user friendly collection.
Stabilize the flora by mixing the sample and the stock solution immediately after taking the sample.
--->Minimize bias introduced by microbial growth and DNA degradation.
It can be stored for 60 days at ambient temperature without freezing, making it easy to manage samples during transportation.
--->It is easier to handle because it saves labor and cost compared to the conventional method, and the sample can be stored at ambient temperature.
Since the bacterial flora at the time of collection can be preserved, samples suitable for metagenomics analysis and flora analysis can be obtained.
--->Minimize noise in your data analysis obtain reliable microbiota profile.
Oragene, ORAcollect and OMNIgene products can be inactivated of SARS-CoV-2.

Comparison with other company’s kits

Feces were collected and stored using OMNIgene･GUT and other company’s kits, and the extracted samples were compared. It was confirmed that the OMNIgene･GUT sample had small variations in the total microbiome (A), and even when the sample was dispensed and divided into multiple samples (B).

What is the gut microbiome?

A human body is made up of all kinds of different cells. Some of these cells are human cells such as skin cells or blood cells. We are also host to trillions of microscopic microbial cells which include bacteria, archaea, fungi, protists and viruses. This group of non-human cells within our bodies is referred to as the human microbiome and they have their own unique set of genes. The gut microbiome refers to the microbes living in our gastrointestinal tract. It is the largest and most diverse microbial community in the human body and scientists are discovering this community plays an important role in our health.

Microbial Collection and Stabilization Kits　OMNIgene®- GUT

The graph compares fresh samples, frozen at -80 ° C samples, OMNIgene GUT samples, and samples not using OMNIgene GUT. With OMNIgene GUT, you can get close results with fresh samples and frozen at -80℃ samples. The results of Doner B show that the balance of the flora is broken in the samples not using OMNIgene GUT compared with other samples.

Reference : Identification of factors that affect the reproducibility and quality of results in analyzing flora profiles.