Cell incubation & Processing system, Xvivo

Biospherix Ltd. (USA)

Features of Cell incubation & Processing system, Xvivo

1. Control temperature, oxygen and CO2 condition

2. Xvivo has the option of multiple independent chambers per incubator. Each can have different conditions simultaneously. That means different culture protocols can run in one incubator at the same time.

3. Easy to access for cell processing

Xvivo System has been warmly embraced by the cell therapy community as an attractive alternative to cleanrooms.

4. High flexibility module design

Cells need more than conventional incubators provide. This cutting edge incubation technology is the first that can satisfy those needs. More than a typical incubator, it is a comprehensive system of modular interconnecting incubation chambers, sub-chambers, and co-chambers with advanced controls. Modules can be fitted together like building blocks, and configured for virtually any application, large or small. If needs change, you can easily modify and upgrade your incubator by adding or removing modules or controls. Your incubator will never again constrain your research. Your investment will never become obsolete. Now do experiments not possible in conventional equipment. Your cells will benefit.

5. GMP Feature

We provide GMP system at user's option. It closely aligned with quality management for cell production

Cell incubation & Processing system Xvivo system schematic

(1) PC provides familiar, convenient interface,　security access, and datalogs everything. PC　accessories allow you to operate system from　any remote location via modem, network, or　internet

(2) to (4) Controller controls wide range of conditions in first cell culture chamber (temperature, CO2, O2, CO, NO). Conditions can be different in each chamber. Conditions can be static ordynamic.

(5) Controller can interface special custom accessories such as liquid sensors in media, timed aliquots of liquid infused into media, fiber optic spectrometry, etc.

(6) Controller duplicates conditions of glove workspace to each different incubator just before it is opened. Optimal conditions need never be disturbed. Workspace atmosphere is constantly HEPA filtered, UV sterilized, activated carbon adsorbed, and dehumidified to levels less than those that support bacterial proliferation.

(7) Controller adapts conditions of pass-through chamber to glove box so conditions in glove box are not affected by moving things in and out.

(8) to (10) Nitrogen is used to push down oxygen and carbon dioxide. Oxygen is used to push up oxygen.. Carbon dioxide is used to push up carbon dioxide.

(11)Transport chambers are part of the system. They can transport cultures uninterrupted and be opened uninterrupted inside glove workspace.

Application

• Tissue engineering：Tissue regeneration breakthrough of mechanism of cell differentiation under near-in vivo environment.
• Cancer treatment：Cancer cell experiment under hypoxia.
• Drug discovery：Effective for drug search process, production control.
• IVF：Quality control fertile egg, for better implantation rate.

Roswell Park Launches Immunotherapy Vaccine Trial Using Xvivo System

(YouTube 3:08)

Developed at RPCI (Roswell Park Cancer Institute), the NY-ESO-1 dendritic cell vaccine

List of abstracts concerning Xvivo Workstation (PDF)

1. Oxygen tension controls the expansion of human CNS precursors and the generation of astrocytes and oligodendrocytes.
2. The effect of oxygen tension on the in vitro assay of human osteoblastic connective tissue progenitor cells.
3. Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure.
4. Hypoxia and HIF1alpa repress the differentiate effects of BMPs in high-grade glioma.
5. IFATS collection: Adipose stromal cells adopt a proangiogenic phenotype under the influence of hypoxia.
6. Continuous hypoxic culturing maintains activation of Notch and allows long-term propagation of human embryonic stem cells without spontaneous differentiation.
7. Transcriptional signature of human adipose tissue-derived stem cells (hASCs) preconditioned for chondrogenesis in hypoxic conditions.
8. Effect of oxygen concentration, culture format and donor variability on in vitro chondrogenesis of human adipose tissue-derived stem cells.
9. The effects of low oxygen on self-renewal and differentiation of embryonic stem cells.

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Manufacturer：Biospherix Ltd. (USA)