3D Cell Culture Plate LifeGel™
LifeGel for 3D cell culture comes as a ready-to-use plate containing a protein-based hydrogel. Cells seeded on the surface of LifeGel can form three-dimensional structures such as spheroids. LifeGel has many advantageous properties compared with competitors...
Feature of LifeGel™
- Protein-based
- Batch to batch repeatable
- Defined hydrogel stiffness
- Easily digestible
- Customisable for different cells and assays
- Supply for small and large users
- Compatible with standard imaging platforms
- Growth factor free
Defined gelatine fraction cross-linked within assay wells, and supplied ready for surface cell seeding.
⇢ Surface stiffness stimulates cells to different morphologies
⇢ Spheroids created on the surface have equal access to tested drugs
Product Line up
3D cell culture LifeGel™
| Product name | LIFEGEL™ FOR 3D CELL CULTURE |
LIFEGEL™ STARTER PACK |
PANEL OF LIFEGELS™ FOR 3D CELL CULTURE |
|---|---|---|---|
| Plate | 48, 96 or384 well plate | 48, 96 or384 well plate | Only 48 well plate |
| Hydrogel | Standard or Customized | Standard or Customized | 16 different hydrogels in triplicate or 24 different hydrogels in duplicate |
| Number of plate | 2 | 2 | 1 |
Digestion Kit
| Product name | LIFEGELTM DIGESTION KIT | TISSUE DIGESTION KIT |
|---|---|---|
| Application | Sufficient for digestion of a hydrogel on one plate | Intendent for digestion of 16 g of tissue |
More than 30 cell lines tested
Various of cell lines have been cultured on the LifeGel:
Human cell lines :
- LoVo - colorectal adenocarcinoma
- LS180 - colorectal adenocarcinoma
- U-2 OS - bone osteosarcoma
- PANC-1 - pancreatic cancer
- MDA-MB-231 – breast cancer
- MCF-7 – breast cancer
- HCC1806 - triple negative breast cancer
- MCF-10A - non-tumorigenic epithelial cell line
- T226 - HER2+ breast cancer xenograft-derived cells
- HBCx-9 - Triple negative breast cancer xenograft-derived cells
- HBCx-3 - ER+ breast cancer xenograft-derived cells
- HUVEC- human umbilical vein endothelial cells (for angiogenesis research)
- Immortalised HUVEC (for angiogenesis research)
- RKO – colon carcinoma
- RCC – renal cell carcinoma
- HEK 293 - embryonic kidney cells
- SK-MEL-2 – malignant melanoma
Hamster cell lines :
- CHO – Chinese hamter ovary cells
Co-culture :
- CHO cells with Jurkat T (human lymphocytes, leukemia )
Murine cell lines :
- Pan_02 - pancreatic ductal adenocarcinoma
- 4T1 – breast cancer
- B16-F10 – skin; melanoma
- L929 – fibroblast
Techniques Applicable for Use with LifeGel™
Cell imaging with professional equipment like for example: JuLI™ Stage, Celloger™ or Opera
- Long term cell culture e.g. three weeks
- Easy cell harvesting due to simple to use enzymatic digestion kit – for cell passaging, cell counting or any other experiments where access to cells is necessary
- Readout your assay on standard plate reader, for example measure viability with CellTiter-Blue® or CellTiter-Glo®
- Immunohistochemistry staining (IHC) Compatible with many types of automatic pipetting equipment
- No prior preparation – just ready to use plates
- We have already created multiple applications and see many more possibilities!
Spheroid formation on the different types of LifeGel™
LifeGel optimised for PANC-1 cell line. Five types of LifeGel were observed to result in different morphology of PANC-1 cells. Physical interaction of cells with various LifeGels caused changes in gene expression profiles and therefore changed morphology of cells.
IC50s for several drugs have been compared in 2D versus 3D cell culture using PANC-1 cells. As commonly observed, 3D cell cultures show less sensitivity to drugs than 2D cell cultures.
Comparable data has been obtained in 384-well LifeGel™ plates (Z’ values 0.7 +/- 0.1). Different assays may be multiplexed, including DNA quantitation.
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| キーワード分類1 | Cell |
|---|---|
| キーワード分類2 | Culture |
| キーワード分類3 | 3D cell culture |
| 製品カテゴリ1 | Device |
| 製品カテゴリ2 | Cell culture |
| 分野 | Drug discovery|Cancer|Regenerative medicine|Organ simulator|Biological imaging|Cell imaging |
| サムネイル画像 |
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| 説明文 | Control precise O2, N2, CO2 gas concentration, could incubate, manipulate, observe your cell. |
| リダイレクトURL |
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