Live Cell Imaging Chamber and Incubation Observation System Chamlide™

What's "Chamlide"?

Chamlide offers culture chamber, incubator system for high magnification microscopy, and peripheral equipment necessary for the Live Cell Imaging (Time lapse Imaging, etc).

What is "Culture Chamber"?
Types of Cultuer Chambers
System for Live Cell Imaging
Application

What's "Culture Chamber"? -- uses a magnet for quick setting

Prevents the breaking of the cover slip and the leaking of medium

The culture chamber is autoclavable and, as such, can be reused many times.
Also, it is extremely economical when compared to expensive and non-reusable commercial dishes and chambers.

The Various Types of Culture Chambers we offer

35mm Dish type 1-well magnetic chambers for round coverslip （CM-BXX-1）
12, 15, 18, 20, 22, 25 Φ coverslip

35mm Dish type 1-well magnetic chambers for round coverslip （CM-BXX-1PA/-1PB）
(Straight or L shape tubing type)
12, 15, 18, 20, 22, 25 Φ coverslip

35mm Dish type multi-well magnetic chambers for round coverslip (CM-M25-2/-4)
(2 wells or 4 wells)
25Φ coverslip

35mm Dish type 4 wells magnetic chambers for round coverslip (CM-M12-4)
12Φ coverslip

35mm Dish type magnetic chambers for square coverslip（CM-SXX-1）
18x18、20x20、22x22、24x24mm

35mm Dish type multi-well magnetic chambers for square coverslip（CM-SXX-2/-4）
18x18、20x20、22x22、24x24mm

Water circulation Magnetic Chamber (WC-RXX)
12、15、18、20、22　25Φ coverslip

Perfusion type water volume adjustable magnetic chamber(AC-BXX)
15、18、20、22　25Φcover slip

Perfusion type water volume adjustable magnetic chamber for patch clump(AC-PXX)
15、18、20、22　25Φcover slip

Perfusion type water volume adjustable magnetic chamber for patch clump minimum surface fluctuation (AC-PS15)

Perfusion type electrical stimulation magnetic chamber（EC-BXX）
15、18、20、22　25Φ coverslip

Perfusion type closed magnetic chamber for shear stress experiment (CF-SXX-A)
15、18、20、22　25Φ coverslip
Height of Internal channel：0.2, 0.5, 1.0mm

Many other different types of chambers, corresponding to one's research goals are also available

Microscope Incubator Stage-top Incubator System T　

Stage-top Incubator System T is an integrated system maintains all essential requiremnts in cultivation and observation of living cells on microscope for the purpose of ‘happiness for the cells and success for researchers’.

It provides cell-friendly environment conditions including optimized temperature, humidity, pH-value and gas concentration for a long-term live cell imaging.

Stage top type Incubator for microscopy

Available with various chamber including 35mm culture dishes and multi-well plate
Temperature of main body, incubator cover, humidifier, lens warmer and gas concentration is controllable and recordable by CCP(Computer Controlled Program) software
Gas gradients are programmable by CCP software
Compatible with various of microscopes
Touch pad control
Magnet attachment cable
Sliding & open incubator cover
Compact size & easy assembly
Custom incubator system is available

Components

Incubator

Maintains an optimal condition including temperature, pH, humidity and CO2 concentration for cell growth
Generates uniform heat by incubator cover and main body
Cover is composed of high transparent dual-layer glass; heating glass & reinforced glass
Body is compatible with all types of culture dishes and multi-well plates
Composed with the whole-opening in main body is compatible with multi-well plates when using high NA(Numerical Aperture) objectives
Provides uniform temperature distribution in well plate(±0.1~0.3℃between wells)

Controller

Utilizes PID Control maintains the temperature of all heating parts including incubator cover, incubator main body, humidifier and lens watmer
Adjusts the flow rate and gas concentration
Supplies O₂,CO₂ and N₂ depend on gas mixer

Humidifier

Keeps saturated humidity in the incubator
Maintains osmolality of medium by preventing evaporation
Flows humid gas into the incubator

Lens-warmer

Helps to keep temperature of the immersion objective lens for high-resolution imaging
Uses magnetic attachment technique prevents cord breakage by tangling or over tension
Fits all types of objectives
With specialized silicon heater wraps the lens without gap

Remote Touch Pad

Controls system and all parameters by smart phone and other devices
Provides on line troubleshooting
Uses GUI(Graphical user interface) with intuitive and easy control

3D Culture System

Collaboration system with 3D Culture Well plate allowing spheroids culture.
Incubators for ASI Piezo Z stage, LUDL, and PI Piezo stages are also available.

Application

GFP / RFP expression
ROS
FRAP

FRET
Time Lapse Imaging
All other types of live cell imaging

An example of how the Chamlide EC Electrically Stimulated Perfusion
Chamber heating plate and fluid inline heater are used

Time lapse imaging of hippocampal neuron cells
EGFP-tagged target protein is moving into dendritic spines by neuronal stimulation. Dr. Sunghoe Chang. Department of Life Science, GIST

An example of how the Chamlide FC Closed
Perfusion Chamber heating plate and fluid inline heater are used

Membrane tubular structures are forming by overexpression of EGFP-tagged target protein into the cells. Dr. Sunghoe Chang. Department of Life Science, GIST

Example of Chamlide TC, 35mm dish type Magnetic Chamber 15hours of time-lapse imaging of a liver cancer cell

(YouTube 1:06)

*D culture sheet(SCIVAX Corporation), Nikon TE 2000 microscope is used

We take orders for special chambers and systems as well. Feel free to contact us.

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キーワード分類1	Cell
キーワード分類2	Culture
キーワード分類3	Imaging
製品カテゴリ1	Device
製品カテゴリ2	Cell culture
分野	Drug discovery\|Cancer\|Regenerative medicine\|Cardiology\|Ophthalmology\|Pediatrics\|Biological imaging\|Cell imaging
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説明文	Live Cell Imaging observation chamber, incubator system
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