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Robotic Isolation Technology CellSorter

CellSorter develops smart technologies for single cell handling, sorting, and isolation. We design flexible hardware and software for diverse applications(Single cell DNA/RNA sequencing, CTC detection or Protein engineering).

Main Features

  • High throughput single cell sorting directly from the Petri dish
  • One single cell arrives to each PCR tube
  • 10 PCR strips containing 80 tubes can be filled in a cycle
  • Glass cover slip for testing single cell deposition in situ
  • Drop volume less than 1 ul for adherent cells
  • Pick up volume of ~1 nl for suspended cells
  • 15-20 seconds per cell
  • Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
  • Both unlabeled and fluorescent cells are recognized by computer vision
  • Viable cells after sorting
  • Sorts cells labeled by fluorescent molecular probes highlighting specific cellular activities
  • Collects single cells for further cultivation, cloning, RNA or protein preparation
  • Any adherent and non-adherent cell type can be sorted
  • Cell culture needs minimal preparation before sorting
  • Average sorting process takes only a few minutes
  • Multichannel detection using the fluorescent filter setup of the microscope
  • When collecting multiple cells sorting speed is 1 cell/second. Number of cells picked up in a single run: 1-1000

New Piezoelectric Sorting Head for Single Cell Isolation

Pipetting nanoliter scale volumes is challenging due to several technical difficulties including surface tension, adhesion and evaporation effects. We developed a fully automated piezoelectric micropipette with a precision of <1 nanoliter. The piezo micropipette can be fully automated using either fluorescent or transparent illumination on a microscope.

Main applications   single cell isolation, single cell printing, and nanoliter droplet printing.

Features

  • Compact design
  • Excellent liquid handling precision: 0.1 nanoliter
  • Outstanding >90% single cell isolation efficiency
  • Built-in LED illumination for phase contrast imaging
  • No tubes
  • No syringes
  • Control unit with USB connection to the computer ​

Robot Picking Single Cells Controlled By High Speed Valves

Features

  • CellSorter control unit with 2 high speed fluid valves and USB connection to the computer
  • High precision micropipette holding console manufactured from hard aluminum equipped with LED illumination for micropipette focusing and connected to the motorized micromanipulator via a rotatable arm.
  • Special aluminum insert fitting to the microscope stage with a hole and magnetic fixing ring for the 35 mm Petri dish and 80 holes for 10 PCR stripes and a hole for a 32x24 mm2 glass cover slide for testing and high resolution imaging and a hole with a calibration cross­hair
  • Accessories, fittings and tubing for liquid control
  • Compatible with most inverted microscopes
  • Modular structure makes it versatile and helps to fit the device to the specific application
Hardware Requirements
  • Inverted microscope with digital camera, motorized focus, and stage
  • Syringe pump

Valve Controlled Cellsorter Device Standard Configuration

Comparison of CellSorter technology with competing methods

  CellSorter  Other Methods  
Valve Control Piezo Head Other robots using micropipettes FACS Droplet Based Devices Manual Pipetting
Imagning Baced Cell Selection ✔ ✔ ✔ X X ✔
Fluorescent Markers Correlanted to the DNA/RNA Sequence ✔ ✔ ✔ ✔ X ✔
Appropriate for Sensitive Cells ✔ ✔ ✔ X ✔ ✔
Appropriate for Cell Suspention X ✔ X ✔ ✔ ✔
Isolation of Rare Cells ✔ ✔ ✔ X X ✔
Installation on Various Microscopes ✔ ✔ X N/A N/A ✔
Low Operation Cost ✔ ✔ ✔ X X X
Low Device Cost ✔ ✔ X X ✔ ✔
Resuced Hands-On Time ✔ ✔ ✔ ✔ ✔ X
Compact Device X ✔ X ✔ ✔ N/A

Scientific reference

Single Cell Isolation and Sorting

  • R. Salánki et al.: High-throughput image based single cell isolation, Microscopy and Analysis, January issue, S10-13 (2015) Open access paper.
  • R. Ungai-Salánki et al.: Automated single cell isolation from suspension with computer vision, Scientific Reports 6, Article number: 20375 (2016) Open access paper.

Single Cell RNA Sequencing

  • M. Ngara et al.: Exploring parasite heterogeneity using single-cell RNA-seq reveals a gene signature among sexual stage Plasmodium falciparum parasites, Experimental Cell Research (2018)
  • Payam Haftbaradaran Esfahani et al.: Cell shape determines gene expression: cardiomyocyte morphotypic transcriptomes, Basic Research in Cardiology 115(7) (2020) ​

Circulating tumor cell (CTC) isolation

  • Marnie Winter et al.: Isolation of Circulating Fetal Trophoblasts Using Inertial Microfluidics for Noninvasive Prenatal Testing, Advanced Materials Technologies 1800066 (2018) ​

Protein Engineering​

  • K. Piatkevich et al. : A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters, Nature Chem Biol, doi:10.1038/s41589-018-0004-9 (2018) ​

Single Cell Adhesion Force

  • N. Sándor et al.: CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18, PLoS ONE 11(9), e0163120 (2016) Open access paper.
  • T. Gerecsei et al.: Adhesion force measurements on functionalized microbeads: An in-depth comparison of computer controlled micropipette and fluidic force microscopy, Journal of Colloid and Interface Science, 555, 245-53 (2019)

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キーワード分類1 Cell
キーワード分類2 Analysis
キーワード分類3
製品カテゴリ1 Device
製品カテゴリ2 Cell analysis
分野 Drug discovery|Cancer|Regenerative medicine|Biological imaging|Genomics|Biomarker|Bioinformatics|Cell imaging|Single cell
サムネイル画像 Robotic Isolation Technology CellSorter
説明文 CellSorter develops smart technologies for single cell handling, sorting, and isolation.
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